- Arroyo City
- Bayview Irrigation District
- Cameron County Drainage District # 3
- Cameron County Drainage District # 5
- City of Alamo
- City of Bayview
- City of Donna
- City of Harlingen
- City of La Feria
- City of La Joya
- City of Laguna Vista
- City of Los Fresnos
- City of McAllen
- City of Mercedes
- City of Mission
- City of Pharr
- City of Point Isabel
- City of Rio Hondo
- City of San Benito
- City of San Juan
- City of Santa Maria
- City of Santa Rosa
- City of Weslaco
- Delta Lake Irrigation District
- Texas A&M AgriLife Extension, District 12
- Edinburg Scenic Wetlands & World Birding Center
- Estero Llano Grande World Birding Center
- Harlingen Irrigation District
- Hidalgo County Irrigation District #2
- Hugh Ramsey Park World Birding Center
- Laguna Atascosa National Wildlife Refuge
- Los Fresnos Nature Park
- Lower Rio Grande Valley Development Council
- Port of Harlingen Authority
- Prairie View A&M University Cooperative Extension, Agriculture and Natural Resources
- Region M Water Planning Group
- Rio Farms Inc.
- Rio Grande Valley Birding Festival
- San Benito Irrigation District
- Santa Ana National Wildlife Refuge
- South Padre Island
- Texas A&M University – Kingsville
- Texas A&M AgriLife Extension Service, Cameron County
- Texas A&M AgriLife Extension Service, Hidalgo County
- Texas A&M AgriLife Extension Service, Willacy County
- Texas Commission on Environmental Quality
- Texas Parks & Wildlife Department
- Texas State Soil & Water Conservation Board
- Texas Water Development Board
- The Meadows Center for Water and the Environment – Texas State University
- U.S. Department of Agriculture – Natural Resource Conservation Service
- U.S. Environmental Protection Agency
- U.S. Fish & Wildlife Service
- U.S. Section of International Boundary & Water Commission
- University of Texas Rio Grande Valley
- Valley Environmental Summit
- Valley Land Fund
- Valley Nature Center
